r d systems cat 10584 cv Search Results


recombinant il 21  (Sino Biological)
94
Sino Biological recombinant il 21
Optimization of single memory B ELISPOT assays (A) Single B cells were sorted using a number of different flow cytometric staining panels and capture media with either 20% or 40% addition of fetal bovine serum. Cells were cultured for 14 d in the presence of <t>IL-21</t> and feeder cells expressing huCD40L. Bars (mean with SD) indicate the number of wells out of a 96-well plate that resulted in expression of IgG as measured by a total IgG ELISA. Results are grouped into a high (>1 .000), medium (0.5–0.999) and low (0.100–0.499) optical density readouts. (B) Supernatants from 2 96-well plates for each condition were pooled and assayed for IgG concentration in an ELISA assay.
Recombinant Il 21, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+d+systems+cat+10584+cv/Human+Interleukin-21+%2F+IL-21+Protein/pmc04966506-256-32-34
Average 94 stars, based on 1 article reviews
recombinant il 21 - by Bioz Stars, 2026-10
94/100 stars
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r d systems cat 10584 cv  (R&D Systems)
93
R&D Systems r d systems cat 10584 cv
Optimization of single memory B ELISPOT assays (A) Single B cells were sorted using a number of different flow cytometric staining panels and capture media with either 20% or 40% addition of fetal bovine serum. Cells were cultured for 14 d in the presence of <t>IL-21</t> and feeder cells expressing huCD40L. Bars (mean with SD) indicate the number of wells out of a 96-well plate that resulted in expression of IgG as measured by a total IgG ELISA. Results are grouped into a high (>1 .000), medium (0.5–0.999) and low (0.100–0.499) optical density readouts. (B) Supernatants from 2 96-well plates for each condition were pooled and assayed for IgG concentration in an ELISA assay.
R D Systems Cat 10584 Cv, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+d+systems+cat+10584+cv/Recombinant+SARS-CoV-2+Spike+S2+Subunit+His-tag+Protein%2C+CF/pmc11893414-31-48-48
Average 93 stars, based on 1 article reviews
r d systems cat 10584 cv - by Bioz Stars, 2026-10
93/100 stars
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antibodies tri methyl histone h3 lys27 c36b11 rabbit mab  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc antibodies tri methyl histone h3 lys27 c36b11 rabbit mab
Optimization of single memory B ELISPOT assays (A) Single B cells were sorted using a number of different flow cytometric staining panels and capture media with either 20% or 40% addition of fetal bovine serum. Cells were cultured for 14 d in the presence of <t>IL-21</t> and feeder cells expressing huCD40L. Bars (mean with SD) indicate the number of wells out of a 96-well plate that resulted in expression of IgG as measured by a total IgG ELISA. Results are grouped into a high (>1 .000), medium (0.5–0.999) and low (0.100–0.499) optical density readouts. (B) Supernatants from 2 96-well plates for each condition were pooled and assayed for IgG concentration in an ELISA assay.
Antibodies Tri Methyl Histone H3 Lys27 C36b11 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+d+systems+cat+10584+cv/Tri-Methyl-Histone+H3+(Lys27)+Rabbit+mAb/pm36867537-35-17-26
Average 95 stars, based on 1 article reviews
antibodies tri methyl histone h3 lys27 c36b11 rabbit mab - by Bioz Stars, 2026-10
95/100 stars
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il 21  (Sino Biological)
93
Sino Biological il 21
Optimization of single memory B ELISPOT assays (A) Single B cells were sorted using a number of different flow cytometric staining panels and capture media with either 20% or 40% addition of fetal bovine serum. Cells were cultured for 14 d in the presence of <t>IL-21</t> and feeder cells expressing huCD40L. Bars (mean with SD) indicate the number of wells out of a 96-well plate that resulted in expression of IgG as measured by a total IgG ELISA. Results are grouped into a high (>1 .000), medium (0.5–0.999) and low (0.100–0.499) optical density readouts. (B) Supernatants from 2 96-well plates for each condition were pooled and assayed for IgG concentration in an ELISA assay.
Il 21, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+d+systems+cat+10584+cv/Human+Interleukin-21+%2F+IL-21+Protein/pm39638793-432-27-30
Average 93 stars, based on 1 article reviews
il 21 - by Bioz Stars, 2026-10
93/100 stars
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mouse anti-human/monkey/cow/cat/mouse muc5ac antibody  (RayBiotech)
N/A
Mouse anti-Human/Monkey/Cow/Cat/Mouse MUC5AC Antibody
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twist separation beads and purification beads  (Twist Bioscience)
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Twist Sep Beads N Purfn Beads
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mouse anti-human/monkey/cow/cat/mouse muc5ac antibody  (RayBiotech inc)
N/A
Mouse anti-Human/Monkey/Cow/Cat/Mouse MUC5AC Antibody
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mouse anti-human/monkey/cow/cat/mouse muc5ac antibody [sodium azide free]  (RayBiotech)
N/A
Mouse anti-Human/Monkey/Cow/Cat/Mouse MUC5AC Antibody [Sodium Azide Free]
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Optimization of single memory B ELISPOT assays (A) Single B cells were sorted using a number of different flow cytometric staining panels and capture media with either 20% or 40% addition of fetal bovine serum. Cells were cultured for 14 d in the presence of IL-21 and feeder cells expressing huCD40L. Bars (mean with SD) indicate the number of wells out of a 96-well plate that resulted in expression of IgG as measured by a total IgG ELISA. Results are grouped into a high (>1 .000), medium (0.5–0.999) and low (0.100–0.499) optical density readouts. (B) Supernatants from 2 96-well plates for each condition were pooled and assayed for IgG concentration in an ELISA assay.

Journal: mAbs

Article Title: Rapid isolation of dengue-neutralizing antibodies from single cell-sorted human antigen-specific memory B-cell cultures

doi: 10.1080/19420862.2015.1109757

Figure Lengend Snippet: Optimization of single memory B ELISPOT assays (A) Single B cells were sorted using a number of different flow cytometric staining panels and capture media with either 20% or 40% addition of fetal bovine serum. Cells were cultured for 14 d in the presence of IL-21 and feeder cells expressing huCD40L. Bars (mean with SD) indicate the number of wells out of a 96-well plate that resulted in expression of IgG as measured by a total IgG ELISA. Results are grouped into a high (>1 .000), medium (0.5–0.999) and low (0.100–0.499) optical density readouts. (B) Supernatants from 2 96-well plates for each condition were pooled and assayed for IgG concentration in an ELISA assay.

Article Snippet: In brief, cells were sorted into 96-well tissue culture plates containing 150 μl of irradiated 293T cells expressing human CD40L (4.0 × 10^4 cells/well), with RPMI media supplemented with 40% FBS and recombinant IL-21 (Sino Biologicals, Cat. No: 10584-HNAE-20) at a final concentration of 50 ng/mL.

Techniques: Enzyme-linked Immunospot, Staining, Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay